mmp13 (Bioss)

Bioss mmp13

Primer sequences used for real-time polymerase chain reaction.

Mmp13, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews

mmp13 - by Bioz Stars, 2026-03

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anti mmp 13 (Bioss)

Bioss anti mmp 13

Anti Mmp 13, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mmp 13/product/Bioss
Average 94 stars, based on 1 article reviews

anti mmp 13 - by Bioz Stars, 2026-03

94/100 stars

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mmp13 (Cusabio)

Cusabio mmp13

Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and <t>MMP13</t> determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.

Mmp13, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp13/product/Cusabio
Average 91 stars, based on 1 article reviews

mmp13 - by Bioz Stars, 2026-03

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bs 23660r rabbit anti mmp13 bioss (Bioss)

Bioss bs 23660r rabbit anti mmp13 bioss

Bs 23660r Rabbit Anti Mmp13 Bioss, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews

bs 23660r rabbit anti mmp13 bioss - by Bioz Stars, 2026-03

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pthrp (OriGene)

OriGene pthrp

Pthrp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pthrp/product/OriGene
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Image Search Results

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Primer sequences used for real-time polymerase chain reaction.

Article Snippet: Sections were then blocked with 10% FBS for 1 h and incubated with primary antibodies against type II collagen (1:2000; mouse monoclonal antibody; Chemi-Con, Temecula, CA), type X collagen (rat polyclonal antibody) (1:200; Cosmo Bio, Tokyo, Japan), COX-2 (rabbit polyclonal antibody) (1:50; Santa Cruz, Europe), PTHrP (rabbit polyclonal antibody) (1:100; OriGene, MD, USA) and MMP13 (1:200; rabbit polyclonal antibody) (1:100; Bioss, MA, USA) at 4 °C overnight.

Techniques:

Celecoxib reduces type X collagen, MMP13 and PTHrP levels and inhibits type II collagen expression in osteoarthritic articular cartilage (A) Immunohistochemical analysis of type X collagen (scale bars: 100 μm) (B) MMP13 (scale bars: 200 μm) (C) type II collagen, and (D) PTHrP (scale bars: 100 μm) in articular cartilage of tibia sections in the OA + Saline and OA + Celec groups. The histogram illustrates the quantification of the positively stained type X collagen and the relative density of MMP13, type II collagen and PTHrP expression in articular cartilage. Each column represents the mean ± SEM of six samples. Data from each group were compared with those of the OA contralateral control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01; the OA + Celec groups compared to the OA + Saline groups # , P < 0.05; ## , P < 0.01.

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Celecoxib reduces type X collagen, MMP13 and PTHrP levels and inhibits type II collagen expression in osteoarthritic articular cartilage (A) Immunohistochemical analysis of type X collagen (scale bars: 100 μm) (B) MMP13 (scale bars: 200 μm) (C) type II collagen, and (D) PTHrP (scale bars: 100 μm) in articular cartilage of tibia sections in the OA + Saline and OA + Celec groups. The histogram illustrates the quantification of the positively stained type X collagen and the relative density of MMP13, type II collagen and PTHrP expression in articular cartilage. Each column represents the mean ± SEM of six samples. Data from each group were compared with those of the OA contralateral control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01; the OA + Celec groups compared to the OA + Saline groups # , P < 0.05; ## , P < 0.01.

Techniques: Expressing, Immunohistochemical staining, Staining

Overexpression of COX-2 increases Col2a1 , Col10a1 , PTHrP , MMP13 and Runx2 transcription (A) Col2a1 (B) Col10a1 (C) PTHrP (D) Ihh (E) MMP13, and (F) Runx2 mRNA expression was measured in vitro in cells transfected with pcDNA3.1-COX-2 and the control pcDNA3-empty vector for 1 and 3 days. GAPDH mRNA was used as the internal control. Each column represents the mean ± SEM of four replicate cultures. Data from each group were compared with those of the control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001 compared to the control culture.

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Overexpression of COX-2 increases Col2a1 , Col10a1 , PTHrP , MMP13 and Runx2 transcription (A) Col2a1 (B) Col10a1 (C) PTHrP (D) Ihh (E) MMP13, and (F) Runx2 mRNA expression was measured in vitro in cells transfected with pcDNA3.1-COX-2 and the control pcDNA3-empty vector for 1 and 3 days. GAPDH mRNA was used as the internal control. Each column represents the mean ± SEM of four replicate cultures. Data from each group were compared with those of the control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001 compared to the control culture.

Techniques: Over Expression, Expressing, In Vitro, Transfection, Plasmid Preparation

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 1. Specific effects of Nrf2 on chondrocyte apoptosis and cellular inflammation (A) Nrf2 overexpression was achieved in chondrocytes by transfecting with Nrf2-overexpressing vector (Nrf2 OE), which was confirmed by western blot analysis. (B,C) Next, chondrocytes were transfected with Nrf2 OE or vector (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h) and examined for cell apoptosis by flow cytometry using an Annexin-V/PI apoptosis kit (B) and TUNEL staining using a TUNEL apoptosis assay kit (C). (D) C-caspase-3, caspase-3, BAX and Bcl-2 were determined by western blot analysis. (E) The mRNA expression levels of IL-6, TNF-α, INOS, and COX2 determined by qRT-PCR. (F) The protein levels of aggrecan, COL2A1, ADAMTS-5, ADAMTS-4, and MMP13 determined by western blot analysis. (G) The protein levels of p-p65 and p65 by determined by western blot analysis. *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+IL-1β group.

Article Snippet: Next, the membranes were incubated overnight, at 4°C with the following antibodies: Nrf2 (CSB-PA003481; Cusabio, Wuhan, China), aggrecan (13880-1-AP; Proteintech), COL2A1 (MA512789; Thermo Fisher), ADAMTS-5 (ab41037; Abcam, Cambridge, USA), MMP13 (CSB-PA07029A0Rb; Cusabio), p-p65 (ab194726 for human; Abcam; CSB-PA000582 for mouse; Cusabio), p65 (10745-1- AP for human; 80979-1-RR for mouse; Proteintech), HMGB1 (CSBPA01604A0Rb; Cusabio), C-caspase-3 (ab32042; Abcam), procaspase-3 (ab32150; Abcam), BAX (50599-2-Ig; Proteintech), Bcl-2 (12789-1-AP; Proteintech), ADAMTS-4 (11865-1-AP; Proteintech), IL-1β (16806-1-AP; Proteintech), actin (66009-1-Ig, Proteintech) and GAPDH (60004-1-Ig; Proteintech), followed by incubation with HRP-conjugated secondary antibody (SA00001-1, SA00001-2; Proteintech) for 1 h at room temperature.

Techniques: Over Expression, Plasmid Preparation, Western Blot, Transfection, Negative Control, Flow Cytometry, TUNEL Assay, Staining, Apoptosis Assay, Expressing, Quantitative RT-PCR

Figure 3. Specific effects of HMGB1 on chondrocyte apoptosis and cellular inflammation (A) HMGB1 knockdown was achieved in chondrocytes by transfecting with small interfering RNA targeting HMGB1 (si-HMGB1), which was confirmed using western blot analysis. Next, chondrocytes were transfected with si-HMGB1 or si-NC (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h). (B) Cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit. (C) The mRNA expressions of IL-6, TNF-α, INOS, and COX2 were examined by qRT-PCR. (D) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis. (E) The protein levels of p- p65 and p65 were examined by western blot analysis. *P<0.05, **P<0.01 vs si-NC group; ††P<0.01 vs si-NC+IL-1β group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 3. Specific effects of HMGB1 on chondrocyte apoptosis and cellular inflammation (A) HMGB1 knockdown was achieved in chondrocytes by transfecting with small interfering RNA targeting HMGB1 (si-HMGB1), which was confirmed using western blot analysis. Next, chondrocytes were transfected with si-HMGB1 or si-NC (negative control) in the presence or absence of IL-1β (10 ng/mL for 24 h). (B) Cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit. (C) The mRNA expressions of IL-6, TNF-α, INOS, and COX2 were examined by qRT-PCR. (D) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis. (E) The protein levels of p- p65 and p65 were examined by western blot analysis. *P<0.05, **P<0.01 vs si-NC group; ††P<0.01 vs si-NC+IL-1β group.

Techniques: Knockdown, Small Interfering RNA, Western Blot, Transfection, Negative Control, Flow Cytometry, Quantitative RT-PCR

Figure 4. Nrf2 acts on chondrocytes through the HMGB1/NFκB pathway (A) Chondrocytes were cotransfected with Nrf2 OE and HMGB1 OE and examined for the protein levels of Nrf2 and HMGB1 using western blot analysis. (B‒E) In the presence of IL-1β stimulation (10 ng/mL for 24 h), cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit (B); the mRNA expression of IL-6, TNF-α, INOS, and COX2 was examined by qRT-PCR (C); the protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis (D); the protein levels of p-p65 and p65 were examined by western blot analysis (E). *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+HMGB1 OE group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 4. Nrf2 acts on chondrocytes through the HMGB1/NFκB pathway (A) Chondrocytes were cotransfected with Nrf2 OE and HMGB1 OE and examined for the protein levels of Nrf2 and HMGB1 using western blot analysis. (B‒E) In the presence of IL-1β stimulation (10 ng/mL for 24 h), cell apoptosis was examined by flow cytometry using an Annexin-V/PI apoptosis kit (B); the mRNA expression of IL-6, TNF-α, INOS, and COX2 was examined by qRT-PCR (C); the protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 were examined by western blot analysis (D); the protein levels of p-p65 and p65 were examined by western blot analysis (E). *P<0.05, **P<0.01 vs vector group; ††P<0.01 vs vector+HMGB1 OE group.

Techniques: Western Blot, Flow Cytometry, Expressing, Quantitative RT-PCR, Plasmid Preparation

Figure 5. Effects of Nrf2 and HMGB1 on OA mouse damage in vivo Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) surgery, and mice were assigned into five groups: (i) Sham, (ii) DMM, (iii) DMM+TBHQ, (iv) DMM+rHMGB1 and (v) DMM+TBHQ+rHMGB1. (A) Histopathological alterations in knee articular cartilage from different groups of mice were verified by H&E staining, scale bar: 100 μm. (B) The expression levels of inflammatory factors (IL-6, TNF-α, iNOS, and COX2) in knee articular cartilage from different groups of mice were determined by ELISA. (C) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 in knee articular cartilage from different groups of mice were examined by western blot analysis. (D) The protein levels of p-p65 and p65 in knee articular cartilage from different groups of mice were examined by western blot analysis. *P<0.05, **P<0.01 vs sham group; ††P<0.01 vs DMM group; ##P<0.01 vs DMM+rHMGB1 group.

Journal: Acta biochimica et biophysica Sinica

Article Title: The Nrf2/HMGB1/NF-κB axis modulates chondrocyte apoptosis and extracellular matrix degradation in osteoarthritis.

doi: 10.3724/abbs.2023078

Figure Lengend Snippet: Figure 5. Effects of Nrf2 and HMGB1 on OA mouse damage in vivo Experimental OA in mice was induced by destabilization of the medial meniscus (DMM) surgery, and mice were assigned into five groups: (i) Sham, (ii) DMM, (iii) DMM+TBHQ, (iv) DMM+rHMGB1 and (v) DMM+TBHQ+rHMGB1. (A) Histopathological alterations in knee articular cartilage from different groups of mice were verified by H&E staining, scale bar: 100 μm. (B) The expression levels of inflammatory factors (IL-6, TNF-α, iNOS, and COX2) in knee articular cartilage from different groups of mice were determined by ELISA. (C) The protein levels of aggrecan, COL2A1, ADAMTS-5, and MMP13 in knee articular cartilage from different groups of mice were examined by western blot analysis. (D) The protein levels of p-p65 and p65 in knee articular cartilage from different groups of mice were examined by western blot analysis. *P<0.05, **P<0.01 vs sham group; ††P<0.01 vs DMM group; ##P<0.01 vs DMM+rHMGB1 group.

Techniques: In Vivo, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Primer sequences used for real-time polymerase chain reaction.

Techniques:

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Techniques: Expressing, Immunohistochemical staining, Staining

Inhibition of COX-2 activity by celecoxib and DFU influences downstream targets in cultured HACs (A) Aggrecan (B) Col2a1 (C) Col10a1 (D) PTHrP , and (E) Ihh mRNA expression in vitro in cells treated with celecoxib and DFU (10 −6 –10 −5 M) for 7 days. GAPDH mRNA was used as the internal control. Each column represents the mean ± SEM of four replicate cultures. Data from each group were compared with those of the control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01 compared to the control culture.

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: Inhibition of COX-2 activity by celecoxib and DFU influences downstream targets in cultured HACs (A) Aggrecan (B) Col2a1 (C) Col10a1 (D) PTHrP , and (E) Ihh mRNA expression in vitro in cells treated with celecoxib and DFU (10 −6 –10 −5 M) for 7 days. GAPDH mRNA was used as the internal control. Each column represents the mean ± SEM of four replicate cultures. Data from each group were compared with those of the control group and were evaluated by one-way ANOVA and Scheffe's test. ∗, P < 0.05; ∗∗, P < 0.01 compared to the control culture.

Techniques: Inhibition, Activity Assay, Cell Culture, Expressing, In Vitro

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Techniques: Over Expression, Expressing, In Vitro, Transfection, Plasmid Preparation

COX-2 promotes PTHrP expression by increasing PTHrP promoter activity and suppressing cell hypertrophy (A) Putative COX-2-binding motifs in the PTHrP reporter. The 5′-flanking region of the human PTHrP gene was subcloned into the pGL3 basic reporter. COX-2 activates PTHrP reporter activity in HACs. pcDNA3.1-COX-2 or pcDNA3.1-empty was cotransfected with the PTHrP (−944 to +50) reporter. The data are presented as the mean ± SE of relative luciferase activity from three independent experiments performed in duplicate. ∗∗, P < 0.01 compared to the pGL3-empty vector (B) qRT-PCR analysis of PTHrP mRNA expression was performed following transfection with pcDNA3-COX-2 and control pcDNA3-empty vector for 1 and 3 days. GAPDH mRNA was used as the internal control. ∗, P < 0.05 compared to the control pcDNA3-empty vector (C) Western blot analysis of PTHrP protein levels digitally detected and normalized to GAPDH levels as a loading control (left panel). Each bar represents the mean ± SEM of three replicate cultures (right panel). Data from the pcDNA3.1-COX-2 vector (hCOX2-ov) group were compared with those of the control pcDNA3.1-empty vector (control vector) group and were evaluated by one-way ANOVA. ∗∗, P < 0.01 compared to the control vector (D) Flow cytometry evaluating the cell size after transfection of cells with pcDNA3.1-COX-2 and control pcDNA3.1-empty vector for 2 and 3 days. Representative results of the mean cell size are shown in the flow cytometry analysis (left panel). Each column represents the mean ± SEM of six replicate cultures. ∗∗∗, P < 0.001 compared to the control culture.

Journal: Journal of Orthopaedic Translation

Article Title: Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats

doi: 10.1016/j.jot.2021.06.003

Figure Lengend Snippet: COX-2 promotes PTHrP expression by increasing PTHrP promoter activity and suppressing cell hypertrophy (A) Putative COX-2-binding motifs in the PTHrP reporter. The 5′-flanking region of the human PTHrP gene was subcloned into the pGL3 basic reporter. COX-2 activates PTHrP reporter activity in HACs. pcDNA3.1-COX-2 or pcDNA3.1-empty was cotransfected with the PTHrP (−944 to +50) reporter. The data are presented as the mean ± SE of relative luciferase activity from three independent experiments performed in duplicate. ∗∗, P < 0.01 compared to the pGL3-empty vector (B) qRT-PCR analysis of PTHrP mRNA expression was performed following transfection with pcDNA3-COX-2 and control pcDNA3-empty vector for 1 and 3 days. GAPDH mRNA was used as the internal control. ∗, P < 0.05 compared to the control pcDNA3-empty vector (C) Western blot analysis of PTHrP protein levels digitally detected and normalized to GAPDH levels as a loading control (left panel). Each bar represents the mean ± SEM of three replicate cultures (right panel). Data from the pcDNA3.1-COX-2 vector (hCOX2-ov) group were compared with those of the control pcDNA3.1-empty vector (control vector) group and were evaluated by one-way ANOVA. ∗∗, P < 0.01 compared to the control vector (D) Flow cytometry evaluating the cell size after transfection of cells with pcDNA3.1-COX-2 and control pcDNA3.1-empty vector for 2 and 3 days. Representative results of the mean cell size are shown in the flow cytometry analysis (left panel). Each column represents the mean ± SEM of six replicate cultures. ∗∗∗, P < 0.001 compared to the control culture.

Techniques: Expressing, Activity Assay, Binding Assay, Luciferase, Plasmid Preparation, Quantitative RT-PCR, Transfection, Western Blot, Flow Cytometry

mmp13 polyclonal antibody Search Results

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